3/9/2024 0 Comments Trimmomatic crop exampleTherefore an upgrade of modulefiles is underway. Files compressed using either „gzip‟ or „bzip2‟ are supported, and are identified by use of „.gz‟ or „.bz2‟ file extensions.ĭue to large number of applications and their versions it is not practical to keep them explicitly listed at our wiki pages. Trimmomatic works with FASTQ files (using phred + 33 or phred + 64 quality scores, depending on the Illumina pipeline used). The paired end mode will maintain correspondence of read pairs and also use the additional information contained in paired reads to better find adapter or PCR primer fragments introduced by the library preparation process. There are two major modes of the program: Paired end mode and Single end mode. These adapters can pose a real problem depending on the library preparation and downstream application. ![]() Trimmomatic is a fast, multithreaded command line tool that can be used to trim and crop Illumina (FASTQ) data as well as to remove adapters.
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